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L-8900 Amino Acid Analyzer

L-8900

Overview - Hitachi's Technology - Software - Specifications

Overview

High Speed Amino Acid Analyzer from Hitachi
Hitachi has manufactured over 1800 Amino Acid Analyzers for over40 years. The L-8900 is capable of analyzing physiological fluids and protein hydrolysates. If you have special requirements, the L-8900 is the one to choose! Hitachi offers the best solution to amino acid analysis.

High Sensitivity: Reaction Column vs. Reaction Coil:
In conventional analyzers, sample zone spreading within the reaction coil causes adverse effects on separation. The L-8900 features a special reaction column (patent pending) to maintain good separation of the sample zone and yet offering high sensitivity and high-speed analysis.

Reaction coil

The speed of the mobile phase is highest at the center of the tube, causing peak broadening.

Reaction Column

By filling the reaction column with beads, the mobile phase moves less smoothly, resulting in a homogeneous flow without peak broadening.

Exceptionally Fast Analysis with Outstanding Detection Limits

Standard amino acid determination takes just 30 minutes (net) and physiological fluid determination can be carried out fully automatically in just 110 minutes.

Front Access
No space is needed at the side of the instrument for maintenance. Just open the front door to replace consumables, columns and the liquid waste container.

No Need for Refrigeration
The L-8900's unique post column delivery design mixes the two ninhydrin reagents just prior to amino acid derivatization. This means they can be stored for a year without refrigeration.

Hitachi's Technology

Automatic Pulsation Dampening

A high-speed, real-time feedback control system ensures excellent flow rate stability. This dual processor system allows small pressure changes to be detected and the pressure adjusted to maintain stability.

Superb Separation with 3Âµm Particles

The finer the packing particles, the higher the number of theoretical plates. That means better separation. Finer particules also boost flow rate at the maximum number of theoretical plates. The 3Âµm packing particles used in the L-8900 are a key technology for fast analysis over a broad range of flow rates with excellent separation.

Detachable Sample Rack

The sample rack may be quickly removed for easy exchange of sample tubes.

Software

EZChrom Elite Chromatography Data System - Expand your capabilities by controlling multiple chromatography systems.

Specifications

Performance (Using Protein Hydrolysate Analysis - 30 min Standard Analysis Method)
Net Analysis Time	30 min
Reproducibility of Retention Time	CV 0.3% (Arg), 0.5% (Ala)
Reproducibility of Peak Area	CV 1.0% (Gly, His)
Detection Limit	3 pmol (S/N = 2, Asp)

Amino Acid Analyzer Main Unit
Column	Size: 4.6 mm i.d. x 60 mm (Resin: 3-µm Hitachi Customer Ion Exchange Resin)
Injection Pump	Pressure: 0 - 20 Mpa (2900 psi) Flow Rate: 0.00 - 0.99 mL/min

Autosampler
Injection Methods	Direct injection method
Sample Vial Capacity	1.5 mL
Number of Sample Vials Accommodated	200 (200 when optional cooler installed)
Injection Volume	0.1 - 100 uL

Column Oven
Method	Peltier
Temperature Setting	20 - 85ºC (in increments of 1ºC)

Reaction Unit
Method	Reaction Column
Temperature Setting	50 - 140ºC (in increments of 1ºC)

Photometer
Spectrometer	Aplanar concave diffraction grating
Wavelength	570 nm, 440 nm

Miscellaneous
Dimensions & Weight (excluding PC)	900 x 650 x 1050 mm ( W x D x H)
Weight (excluding PC)	Approximately 250 kg
Operating Temperature	15-35°C
Power Requirements	115V, 220V ±10%, 1500VA (50/60 Hz)
Data processor	3.2GHz PC 17-inch flat monitor CD-RW/DVD 3.5" floppy drive Mouse and keyboard Microsoft Windows® 2000 or XP Requires EZChrom Elite™ software

Ordering Information
L8900-PH	Protein Hydrolysate Analysis System
L8900-PF	Physiological Fluid Analysis System

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