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L-8900 Amino Acid Analyzer


Overview - Hitachi's Technology - Software - Specifications



High Speed Amino Acid Analyzer from Hitachi
Hitachi has manufactured over 1800 Amino Acid Analyzers for over40 years. The L-8900 is capable of analyzing physiological fluids and protein hydrolysates. If you have special requirements, the L-8900 is the one to choose! Hitachi offers the best solution to amino acid analysis.

High Sensitivity: Reaction Column vs. Reaction Coil:
In conventional analyzers, sample zone spreading within the reaction coil causes adverse effects on separation. The L-8900 features a special reaction column (patent pending) to maintain good separation of the sample zone and yet offering high sensitivity and high-speed analysis.

Reaction coil

before       after

The speed of the mobile phase is highest at the center of the tube, causing peak broadening.

Reaction Column


By filling the reaction column with beads, the mobile phase moves less smoothly, resulting in a homogeneous flow without peak broadening.

Exceptionally Fast Analysis with Outstanding Detection Limits

before       after

Standard amino acid determination takes just 30 minutes (net) and physiological fluid determination can be carried out fully automatically in just 110 minutes.


Front Access
No space is needed at the side of the instrument for maintenance. Just open the front door to replace consumables, columns and the liquid waste container.

No Need for Refrigeration
The L-8900's unique post column delivery design mixes the two ninhydrin reagents just prior to amino acid derivatization. This means they can be stored for a year without refrigeration.


Hitachi's Technology

Automatic Pulsation Dampening


A high-speed, real-time feedback control system ensures excellent flow rate stability. This dual processor system allows small pressure changes to be detected and the pressure adjusted to maintain stability.
Superb Separation with 3µm Particles


The finer the packing particles, the higher the number of theoretical plates. That means better separation. Finer particules also boost flow rate at the maximum number of theoretical plates. The 3µm packing particles used in the L-8900 are a key technology for fast analysis over a broad range of flow rates with excellent separation.


Detachable Sample Rack


The sample rack may be quickly removed for easy exchange of sample tubes.



EZChrom Elite Chromatography Data System - Expand your capabilities by controlling multiple chromatography systems.



Performance (Using Protein Hydrolysate Analysis - 30 min Standard Analysis Method)
Net Analysis Time 30 min
Reproducibility of Retention Time CV 0.3% (Arg), 0.5% (Ala)
Reproducibility of Peak Area CV 1.0% (Gly, His)
Detection Limit 3 pmol (S/N = 2, Asp)
Amino Acid Analyzer Main Unit
Column Size: 4.6 mm i.d. x 60 mm
(Resin: 3-µm Hitachi Customer Ion Exchange Resin)
Injection Pump Pressure: 0 - 20 Mpa (2900 psi)
Flow Rate: 0.00 - 0.99 mL/min
Injection Methods Direct injection method
Sample Vial Capacity 1.5 mL
Number of Sample Vials Accommodated 200 (200 when optional cooler installed)
Injection Volume 0.1 - 100 uL
Column Oven
Method Peltier
Temperature Setting 20 - 85ºC (in increments of 1ºC)
Reaction Unit
Method Reaction Column
Temperature Setting 50 - 140ºC (in increments of 1ºC)
Spectrometer Aplanar concave diffraction grating
Wavelength 570 nm, 440 nm
Dimensions & Weight (excluding PC) 900 x 650 x 1050 mm ( W x D x H)
Weight (excluding PC) Approximately 250 kg
Operating Temperature 15-35°C
Power Requirements 115V, 220V ±10%, 1500VA (50/60 Hz)
Data processor 3.2GHz PC
17-inch flat monitor
3.5" floppy drive
Mouse and keyboard
Microsoft Windows® 2000 or XP
Requires EZChrom Elite™ software
Ordering Information
L8900-PH Protein Hydrolysate Analysis System
L8900-PF Physiological Fluid Analysis System


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